By M. L.; Edsall, John T. (Eds) Anson
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Extra info for Advances in protein chemistry Volume 2
Proline phosphotungstate is somewhat insoluble, and has been used (328) for purifying proline. FEavianic Acid This acid has been employed for precipitating arginine with considerable success. It was introduced by Kossel and Gross (329; cf. 330), who also studied the properties of the flavianates of a number of other bases. It has found widespread use as an almost quantitative precipitant of arginine from complete protein hydrolyzates as its monoflavianate, and in the later stages of determining arginine by other methods.
We propose to use ianophmesis to describe processes concerned with the 32 A. J. P. MARTIN AND R. L. 116. field of relatively small ions, electrophmesis for movement of large molecules and particles and electrodidysis for the removal of smaller ions from large molecules and particles. and electrodialysis only because of the similarity of the apparatus sometimea employed. A. Loddesol (269) has retiewed the use of electrodialysis for soil analysis: Other reviews (270, 271) cover electrophoresis and electrodialysis.
Both middle and cathode compartments are maintained acid. Under these conditions deamination of amino acids occurs at the cathode unless the current density there is kept very low. Only the amino acids go to the cathode compartment. Gordon, Martin, and Synge (282, 283) have used the 3-compartment cell to study the basic andneutral fractions of partial hydrolyzates of proteins. A number of other workers have used the same arrangement (284-289s). Ruppel (290, 291) and colleagues, using chromed gelatin as anode membrane and parchment paper as cathode membrane, used a 3-compartment cell to electrodialyze electrolytes from serum.