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By Rodolfo Paoletti; David Kritchevsky

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The accuracy of both methods was checked using numerous pure lipid standards of known MP. The normal secretion is therefore completely melted as it spreads over the tear film on leaving the meibomian orifice, and, except perhaps under extreme climatic conditions or where blinking is reduced by loss of corneal sensitivity, remains fluid over the normal range of eye surface temperatures Cv32 to 36°C, Alió and Padrón, 1982). The chalazion lipids studied are not, of course, released into the eye; the measurements show principally the effect which alteration of lipid composition could have on the ability of meibomian lipid to melt, leave the glands, and spread on the eye.

Realizing that the temperature of measurement should be that of the surface of the eye, and that the surface layer of lipid would be lost by withdrawing tears, he placed on the eye a scierai contact lens with a circular groove to collect tears and surface lipid, into which a du Noüy ring probe could be suspended. The force required to lift the ring from the liquid was compared with that using water at the same temperature, to compensate for meniscus curvature within the groove. 1°C was calculated for tears.

These workers also investigated the kinetics and cofactor requirements of the synthetic system in some detail. Kolattukudy et al. (1985) also studied the incorporation of radioactively labeled acetate by isolated bovine meibomian glands, including the distribution of radioactivity in different lipid classes, and the chain lengths within these classes, using TLC and radio-GLC. They found 61 % of the total label in WE and SE fractions, distributed equally between acid and alcohol moieties. In WE, the major labeled acids were a-Cis, n-C16, a-C17, and n-C18 : 1, and the major alcohols were a-C23, a-C25, and a-C27 with significant amounts also of i-C24 and i-C26.

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